Fakta om projektet
Ansvarig på SVA
2009 - 2011
Epizootic ulcerastive syndrome, utveckling och implementering av diagnostiska metoder
Epizootic ulcerative syndrome (EUS), caused by the oomycete Aphanomyces invadans (A. piscicida), is a serious emerging notifiable exotic disease, diagnosed in >60 fish species of various families in Asia, Australia, N-America, and Africa. There is no therapy against EUS, and no vaccine. All EU NRL’s for Fish Diseases must have EUS diagnosis in place (ref. 2006/88/EC). Although some tests for EUS were described in literature, most tests were not validated.
Aim/method: The aim of this project was to build up knowledge to recommended confirmatory methods for EUS into the EU:
- Try out the described EUS methods (fresh smears, fungus isolation, histopathology, PCR), choose methods for validation, and write Operational Procedures (OP’s)
- Develop if needed alternative methods (e.g. other agars for isolation, real-time PCR), and validate and implement them.
„h Establish an electronically available slide collection for EUS histopathology.
„h Write OP’s for the recommended diagnostic tests for EUS based on our findings.
„h Evaluate methods for storage, survival of A.invadans outside the host and suitable disinfection.
The four labs kindly received two reference strains from Prof. Kanchanakhan (OIE Reference laboratory in Bangkok), and from Prof. Hatai, Japan.
Results/value of this project (2010-2011):
„h OP´s for 1) selection of fish to sample, 2) sampling for isolation, 3) sampling for PCR & histopathology, 4) cultivation of isolates of A.invadans, 5) sporulation of A.invadans, 6) (q)PCR’s for EUS, and 7) histopathology of EUS. These are illustrated with pictures.
„h An electronically available slide collection for EUS histopathology.
„h Recommendations on storage, survival of A.invadans outside the host and disinfection.
„h At least 4 NRLs being able to diagnose EUS, as examples for the further EU members.
The output (OP’s, slide collection, recommendations) can be used by the NRL’s for Fish Diseases of Europe, and will be accessible via the EURL Fish Diseases website (www.eurl-fish.eu).